Title:Study of Antihypertensive Activity of Anvillea radiata in L-Name-Induced Hypertensive Rats and HPLC-ESI-MS Analysis
Volume: 20
Issue: 7
Author(s): Mourad Akdad, Mohammed Ajebli, Andrea Breuer, Farid Khallouki, Robert W. Owen and Mohamed Eddouks*
Affiliation:
- Faculty of Sciences and Techniques Errachidia, Moulay Ismail University of Meknes, BP 509, Boutalamine, 52000. Errachidia,Morocco
Keywords:
Antihypertensive, vasorelaxation, Anvillea radiata, calcium channels, medicinal plant, direct nitric oxide.
Abstract:
Objective: This study aimed to evaluate the effect of the aqueous extract of Anvillea radiate
(A. radiata) aerial parts (AEAR) on arterial blood pressure in normotensive and hypertensive rats.
Methods: The effect of the acute and sub-chronic administration of AEAR on the following blood
pressure parameters: systolic blood pressure (SBP), mean blood pressure (MBP), diastolic blood pressure
(DBP), and heart rate (HR) was evaluated in normotensive and L-NAME induced hypertensive
rats. In the second experiment, the vasorelaxant effect of AEAR was assessed in isolated aortic rings
from rats with functional endothelium pre-contracted with epinephrine (EP) or KCl, and six antagonists/
inhibitors were used to explore the mechanisms of action involved in the vasorelaxant effect. In
order to determine the phytochemical contents of Anvillea radiata, HPLC-ESI-MS analysis was conducted.
Results: Daily oral administration of AEAR (100 mg/kg) provoked a significant decrease in SBP,
MBP, and DBP without affecting HR in hypertensive rats. In addition, AEAR (0.08-0.64 mg/ml) revealed
a vasorelaxant effect in thoracic aortic rings pre-contracted by EP (10 μM) or KCl (80 mM).
This effect was reduced in the presence of Nifedipine, L-Name or Methylene blue. The polyphenolic
compounds of AEAR were determined.
Conclusion: This study revealed that AEAR possesses a potent antihypertensive activity and its
vasorelaxant activity seems to be mediated through Ca2+ channels, direct nitric oxide (NO), and
NO/cGMP pathways. Chlorogenic acid and caffeic acid identified in A. radiata could be at least partially
responsible for the antihypertensive activity of this extract.