Title:Curcumin Prevents Brain Damage and Cognitive Dysfunction During Ischemic-reperfusion Through the Regulation of miR-7-5p
Volume: 16
Issue: 5
Author(s): Hui Xu, Beibei Nie, Lamei Liu, Chunhui Zhang*, Zhenxiang Zhang, Mengya Xu and Yongxia Mei
Affiliation:
- Department of Clinical Nursing, The Nursing & Health College of Zhengzhou University, Zhengzhou City, 450001,China
Keywords:
Cerebral ischemia-reperfusion, oxygen-glucose deprivation/reoxygenation model, curcumin, miR-7-5p/RelA p65
axis, middle cerebral artery occlusion, tumor necrosis factor.
Abstract:
Objective: This study was to investigate the potential protective effects of curcumin in
cerebral ischemia-reperfusion (CIR) and its regulation of miR-7.
Methods: Rats were occluded by middle cerebral artery occlusion (MCAO) for 1.5 h and reperfused
for 2 h to establish a local CIR model. After 24 hours of model establishment, MCAO rats
were given curcumin for 3 days by intragastric administration. PC12 cells were cultured for 6 h in
oxygen-glucose deprivation medium and then reoxygenated for 24 h to establish an oxygenglucose
deprivation/reoxygenation (OGD/R) model. The OGD/R model cells were treated with
curcumin for 48 h.
Results: Curcumin inhibited the decrease of miR-7-5p expression and an increase of RelA p65 expression
induced by CIR and ODG/R. RelA p65 was a target of miR-7-5p. MiR-7-5p antagonists
were able to counteract the effect of curcumin on the expression of RelA p65 in ischemic brain tissue
of MCAO rats and OGD/R model cells. Curcumin improved OGD/R-induced inhibition of cell
activity, necrosis and apoptosis. Curcumin significantly reduced the levels of tumor necrosis factor
(TNF)-α, interleukin (IL)-6, IL-1β, reactive oxygen species (ROS) and malondialdehyde (MDA)
and increased the activity of superoxide dismutases (SOD) and catalase (CAT) in OGD/R-induced
cells. Curcumin may inhibit OGD/R-induced cell damage by regulating miR-7-5p. Curcumin improved
cerebral infarction, nerve damage and cognitive dysfunction in rats with CIR, which may
be related to the regulation of miR-7-5p/RelA p65 axis.
Conclusion: Curcumin exerts cerebral protection by attenuating cell necrosis and apoptosis, inflammatory
response and oxidative stress following CIR, which may be related to its regulation of
the miR-7/RELA p65 axis.
