Title:The Antitumor Activity of a Novel Fluorobenzamidine against Dimethylhydrazine- Induced Colorectal Cancer in Rats
Volume: 20
Issue: 4
Author(s): Mohammed Abdel-Rasol, Nadia M. El-Beih, Shaymaa M.M. Yahya, Mohamed A. Ismail and Wael M. El-Sayed*
Affiliation:
- Department of Zoology, Faculty of Science, University of Ain Shams, Abbassia 11566, Cairo,Egypt
Keywords:
Colorectal cancer, bithiophene, cisplatin, apoptosis, antioxidants, nephrotoxicity, hepatotoxicity.
Abstract:
Background: Colorectal cancer is among the leading causes of death worldwide. The incidence of
deaths is expected to be 11.4 million in 2030.
Objective: We aimed to evaluate the in vitro and in vivo antioxidant and antitumor activities of a novel Bithiophene-
Fluorobenzamidine (BFB) against DMH-induced colorectal cancer in rats.
Methods: The antiproliferative activity of BFB against HCT-116 colon cancer cells and apoptotic genes was
assessed. In vivo study was also conducted in which 80 adult male rats were divided into 5 groups; control, BFB,
and the other 3 groups were injected with DMH (20mg/kg, s.c., for 9 weeks). Group 4 was injected with 5 doses
of cisplatin (2.5mg/kg, i.p over 21 weeks) and group 5 was injected with 3 doses/week of BFB (2.5mg/kg, i.p,
for 21 weeks).
Results: BFB exhibited weak to moderate in vitro antioxidant activity. It had a strong antiproliferative activity
with IC50 ~0.3µg/ml. BFB induced extrinsic apoptosis through the upregulation of FasL, TRAL, p53 and
caspase-8, and intrinsic apoptosis through the downregulation of Bcl-2 and survivin. BFB decreased the tumor
incidence, multiplicity and size and improved the decreased body weight. BFB also ameliorated the functions of
kidney and liver and antioxidants deteriorated by DMH. BFB significantly improved the pathological changes
caused by DMH in colon tissues.
Conclusion: BFB showed a very promising antitumor activity against colorectal cancer induced by DMH in rats
without causing hepato- or nephrotoxicity.
