Title:Microbial Routes to (2R,3R)-2,3-Butanediol: Recent Advances and Future Prospects
Volume: 17
Issue: 21
Author(s): Neng-Zhong Xie*, Xian-Rui Chen, Qing-Yan Wang, Dong Chen, Qi-Shi Du, Guo-Ping Zhou*Ri-Bo Huang*
Affiliation:
- National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, 530007, Guangxi,China
- National Engineering Research Center for Non-food Biorefinery, State Key Laboratory of Non-food Biomass Energy and Enzyme Technology, Guangxi Biomass Industrialization Engineering Institute, and Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, No. 98 Daling Road, Nanning, 530007, Guangxi,China
- National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, 530007, Guangxi,China
Keywords:
(2R, 3R)-2, 3-Butanediol, Microbial cell factory, Rational design, Metabolic engineering, Synthetic biology, Systematic
and Integrative strategy.
Abstract: (2R,3R)-2,3-Butanediol has many industrial applications, such as it is used as an antifreeze
agent and low freezing point fuel. In addition, it is particularly important to provide chiral groups in
drugs. In recent years, this valuable bio-based chemical has attracted increasing attention, and significant
progress has been made in the development of microbial cell factories for (2R,3R)-2,3-butanediol
production. This article reviews recent advances and challenges in microbial routes to (2R,3R)-2,3-
butanediol production, and highlights the metabolic engineering and synthetic biological approaches
used to improve titers, yields, productivities, and optical purities. Finally, a systematic and integrative
strategy for developing high-performance microbial cell factories is proposed.
