In vitro Synergy Testing of Anidulafungin with Fluconazole, Tioconazole, 5-Flucytosine and Amphotericin B against some Candida spp.

Antonio      Rosato; Monica      Piarulli; Brigida      Immacolata Pia Schiavone; Maria      Teresa Montagna; Giuseppina      Caggiano; Marilena      Muraglia; Addolorata      Carone; Carlo      Franchini; Filomena      Corbo

doi:10.2174/157340612801216184

Abstract

In this paper the authors investigated a synergistic antimycotic effect between four antifungal drugs Amphotericin B, Fluconazole, Tioconazole, and Flucytosine individually combined with Anidulafungin compound. This latter is considered a drug of choice in the treatment of fungal infections; it has good activity both in vitro and in vivo against yeasts and moulds, as Candida and Aspergillus. The goal of this study was to evaluate the in vitro interaction of Anidulafungin in the synergic combinations with previous reported drugs against 12 Candida strains according to CLSI M27-A3 protocol. A synergistic interaction was observed against the most antifungal strains; in particular an increasing of the antimycotic efficacy was obtained from the association between Anidulafungin and Amphotericin B or Fluconazole (Mixture 4:6). In contrast the association Tioconazole/Anidulafungin was less effective on fungal species growth. The antimycotics MIC reduction values were more evident against some strains as C. glabrata, C. krusei, C. tropicalis and C. parapsilosis.

Keywords: Amphotericin B, Anidulafungin, antifungal drugs, C. albicans, non- C. albicans, synergism, synergistic antimycotic, C. tropicalis, Candida and Aspergillus, C. parapsilosis