Abstract
Identification of biomolecule interactions is crucial in understanding biological and biochemical processes. This review presents a brief outline of the advances in microfluidic chips devoted to biomolecule interactions. Various separation modes used for biomolecule interactions are discussed first, in which microchip isoelectric focusing, microchip zone electrophoresis, microchip electrophoresis frontal analysis, and T-sensor microfluidic channel are included. Detection methods on these microsystems are essential for the identification and quantification of chemical species and biomolecule that are being analyzed. Within the detection methods section, time-resolved resonance raman spectroscopy, mass spectrometry, surface plasmon resonance imaging, deep UV laser-induced fluorescence, indirect laser-induced fluorescence, contactless conductivity detection techniques are discussed. Applications including biomolecule interaction kinetics, protein-protein interaction, protein-DNA interaction, drug-protein interaction, and drug-DNA interaction are outlined. To our knowledge, biomolecule interactions studying microfluidic chip have not been reviewed before.
Keywords: Review, Protein-protein interaction, Protein-DNA interaction, Drug-protein interaction, Microchip electrophoresis, Microfluidic chip
Current Pharmaceutical Analysis
Title: Biomolecule Interactions Studying on Microfluidic Chip
Volume: 5 Issue: 2
Author(s): Li-Ping Yu, Yu-Zhen Sun and Zhu-Xuan Zhao
Affiliation:
Keywords: Review, Protein-protein interaction, Protein-DNA interaction, Drug-protein interaction, Microchip electrophoresis, Microfluidic chip
Abstract: Identification of biomolecule interactions is crucial in understanding biological and biochemical processes. This review presents a brief outline of the advances in microfluidic chips devoted to biomolecule interactions. Various separation modes used for biomolecule interactions are discussed first, in which microchip isoelectric focusing, microchip zone electrophoresis, microchip electrophoresis frontal analysis, and T-sensor microfluidic channel are included. Detection methods on these microsystems are essential for the identification and quantification of chemical species and biomolecule that are being analyzed. Within the detection methods section, time-resolved resonance raman spectroscopy, mass spectrometry, surface plasmon resonance imaging, deep UV laser-induced fluorescence, indirect laser-induced fluorescence, contactless conductivity detection techniques are discussed. Applications including biomolecule interaction kinetics, protein-protein interaction, protein-DNA interaction, drug-protein interaction, and drug-DNA interaction are outlined. To our knowledge, biomolecule interactions studying microfluidic chip have not been reviewed before.
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Cite this article as:
Yu Li-Ping, Sun Yu-Zhen and Zhao Zhu-Xuan, Biomolecule Interactions Studying on Microfluidic Chip, Current Pharmaceutical Analysis 2009; 5 (2) . https://dx.doi.org/10.2174/157341209788172915
DOI https://dx.doi.org/10.2174/157341209788172915 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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