Western blotting, also known as immunoblot, is a chromatography-based
technique in which quantitative detection of proteins can be performed based on their
molecular weight. This is often performed after the SDS-PAGE technique. It is used to
identify, analyze and quantify proteins from a protein mixture. The major steps include
1. Separation of proteins (e.g., SDS-PAGE), 2. Transfer onto a solid support, 3.
Utilizing primary and secondary antibodies for visualization. Western blotting can be
used qualitatively and quantitatively. In this chapter, both of these techniques are
explained in detail.
Keywords: Chromatography, Immunoblot, Protein, SDS-PAGE.
