Capillary Electrophoresis (CE) is a powerful separation and analysis
technique that has been rapidly progressing since it was first introduced. The
application range of CE is so diverse that it ranges from small analytes to large and
complex macromolecules. This chapter aims to provide a deep understanding of the
basic principles of CE. The first part of the chapter involves the theoretical basis,
instrumentation, and separation mechanism of CE. The second part focuses on capillary
electrophoretic separation modes and the third part describes the detection methods in
CE. The fourth and final part covers capillary electrophoretic strategies for specific
analyte groups.
Keywords: Capillary electrophoresis, CEC, CGE, Chiral separation, Contactless conductivity detector, CZE, Indirect detection, LIF, MEKC, Proteins, Sample stacking, Small anions, Small organic acids.
