Protocols used in Molecular Biology

Culture and Maintenance of Human Ovarian Carcinoma Cells for Scrutinizing Anti-cancerous Activities of Various Compounds via Some Potent Molecular Markers

Author(s): Juhi Rais, Asif Jafri, Madhu Tripathi and Md Arshad

Pp: 120-125 (6)

DOI: 10.2174/9789811439315120010015

* (Excluding Mailing and Handling)


Ovarian carcinoma is the 5th most common type of cancer of gynecologic origin and accounts for about one-fourth of the total malignancies of the female genital tract. Ovarian carcinoma accounts for highest mortality in females due to the development of chemo-resistance against drugs and lack of symptoms and undetectable biomarkers in the early stages of diagnosis. Tumour debulking, chemotherapies, radiotherapies, targeted therapies, immunotherapies and stem cell transplants are some of the measures that have been adopted by the experts for curing the disease but still, full control over the problem has not been achieved. Research on various herbal and chemosynthetic nano-compounds have shown a new light in this regard, as the studies on them so far have revealed that they have anti-proliferative and apoptotic properties that will help in finding new ways to develop drugs for cancer patients. This chapter deals how to culture and maintain the human ovarian carcinoma cell lines in the laboratory which are being procured from cell repositories and then to study the anticancer efficacy of various promising compounds by potent molecular markers like cellcycle progression and annexin V- FITC apoptosis detection.

Keywords: Anti-proliferative properties, Annexin V-FITC, Nano-compound, Apoptosis, Anti-cancer efficacy, Cancer cell line, Cell-cycle progression, Molecular markers, Ovarian carcinoma.

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