UV Vis spectrophotometry is one of the basic analytical techniques used for the analysis of many inorganic and organic molecules. It is also used for the determination of complex mixtures to some extent. UV Vis spectrophotometry is used for both industrial as well as academic researches. This chapter includes determination in adulteration of some drugs by using this versatile technique. This chapter provides description of research related to the adulteration analysis of drugs by UV spectrophotometry. The chapter also contains a brief discussion about this technique initially followed by published applications in the field of adulteration analysis.