Title:3,6-dichloro-1,2,4,5-Tetrazine Assayed at High Doses in the Metastatic Breast Cancer Cell Line MDA-MB-231 Reduces Cell Numbers and Induces Apoptosis
Volume: 16
Issue: 4
Author(s): Mohamed Abdelkarim*, Khaoula Ben Younes, Ines Limam, Refka Guermazi, Amel Ben Ammaar ElGaaied and Fatma Ben Aissa-Fennira
Affiliation:
- Laboratory of onco-hematology, Faculty of medicine of Tunis, Tunis El Manar University, Tunis,Tunisia
Keywords:
1, 2, 4, 5-tetrazine derivatives, in vitro experiment, MDA-MB-231 cell line, breast cancer, 3, 6-dichloro-1, 2, 4, 5-
tetrazine, apoptosis.
Abstract:
Background: The lack of treatment options for patients with chemotherapy-resistant cancers
is pushing the field toward the development of new therapies. 1,2,4,5-tetrazine derivatives are a class of
heterocyclic compounds that exhibit a broad spectrum of antitumor activities.
Objective: The purpose of this study was to assess the biological activity of four s-tetrazine derivatives
by substitution of two chloride atom of 3,6-dichloro-1,2,4,5-tetrazine with long hydrophobic side
chains.
Methods: We analyzed the anti-proliferative effects of four s-tetrazine derivatives with MTT assay and
their pro-apoptotic effect with AV/ IP flow cytometry analysis and Hoechst 33342 staining.
Results: We demonstrated that 3,6-dichloro-1,2,4,5-tetrazine (compound (1)) has a cytotoxic effect and
induces apoptosis.
Conclusion: 3,6-dichloro-1,2,4,5-tetrazine presents a new cytotoxic drug against metastatic breast cancer
cell line MDA-MB-231 in vitro.
