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Abstract
The evolution of molecular biology has enabled the exploration of novel sophisticated genedirected treating modalities for cancer. Suicide gene therapy - i.e. transfection of a so-called suicide gene that sensitizes target cells towards a prodrug - may offer an attractive approach to treat malignant tumors. For the development of effective clinical suicide gene therapy protocols, a non-invasive method to assay the extent, the kinetics and the spatial distribution of transgene expression is essential. This would allow investigators and physicians to assess the efficiency of experimental and therapeutic gene transfection protocols and would enable early prognosis of therapy outcome. Radionuclide imaging techniques like single photon emission computed tomography (SPECT) and positron emission tomography (PET), which can non-invasively visualize and quantify metabolic processes in vivo, are being evaluated for repetitive monitoring of transgene expression in living animals and humans. Transgene expression can be monitored directly by imaging the expression of the therapeutic gene itself, or indirectly using a reporter gene that is coupled to the therapeutic gene. Various radiopharmaceuticals have been developed and are now being evaluated for imaging of transgene expression. This review surveys the progress that has been made in the field of non-invasive nuclear imaging of transgene expression and focuses on the herpes simplex virus type 1 thymidine kinase (HSVtk) gene therapy approaches.
Keywords: suicide gene therapy, spect, scintigraphic imaging, hsvtr gene therapy
Current Pharmaceutical Design
Title: Scintigraphic Imaging of HSVtk Gene Therapy
Volume: 8 Issue: 16
Author(s): Erik F.J. de Vries, Anne Rixt Buursma, Geke A.P. Hospers, Nanno H. Mulder and Willem Vaalburg
Affiliation:
Keywords: suicide gene therapy, spect, scintigraphic imaging, hsvtr gene therapy
Abstract: The evolution of molecular biology has enabled the exploration of novel sophisticated genedirected treating modalities for cancer. Suicide gene therapy - i.e. transfection of a so-called suicide gene that sensitizes target cells towards a prodrug - may offer an attractive approach to treat malignant tumors. For the development of effective clinical suicide gene therapy protocols, a non-invasive method to assay the extent, the kinetics and the spatial distribution of transgene expression is essential. This would allow investigators and physicians to assess the efficiency of experimental and therapeutic gene transfection protocols and would enable early prognosis of therapy outcome. Radionuclide imaging techniques like single photon emission computed tomography (SPECT) and positron emission tomography (PET), which can non-invasively visualize and quantify metabolic processes in vivo, are being evaluated for repetitive monitoring of transgene expression in living animals and humans. Transgene expression can be monitored directly by imaging the expression of the therapeutic gene itself, or indirectly using a reporter gene that is coupled to the therapeutic gene. Various radiopharmaceuticals have been developed and are now being evaluated for imaging of transgene expression. This review surveys the progress that has been made in the field of non-invasive nuclear imaging of transgene expression and focuses on the herpes simplex virus type 1 thymidine kinase (HSVtk) gene therapy approaches.
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Cite this article as:
de Vries F.J. Erik, Buursma Rixt Anne, Hospers A.P. Geke, Mulder H. Nanno and Vaalburg Willem, Scintigraphic Imaging of HSVtk Gene Therapy, Current Pharmaceutical Design 2002; 8 (16) . https://dx.doi.org/10.2174/1381612023394430
DOI https://dx.doi.org/10.2174/1381612023394430 |
Print ISSN 1381-6128 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4286 |
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