Title:An Overview on Analytical Methods for Quantitative Determination of Aristolochic Acids
Volume: 16
Issue: 5
Author(s): Mohammad-Reza Afshar-Mogaddam, Adeleh Yadeghari and Abolghasem Jouyban*
Affiliation:
- Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz,Iran
Keywords:
Aristolochic acids, capillary electrophoresis, high-performance liquid chromatography, sample preparation, ultraperformance
liquid chromatography, review.
Abstract:
Background: Aristolochic acids are chemically linked to nitrophenanthrene carboxylic acids
which are found in aristolochia plants. These compounds are intrinsically carcinogenic, while they
have been used in traditional medicine from a long time ago. Despite the beneficial effects of herbals
for treating some diseases, they possess some side effects.
Methods: Therefore, the development of a sensitive and selective procedure for the determination of
these harmful components in various complicated samples is an important task for health systems and
drug authorities. In the past years, ultra-pressure liquid chromatography, high performance liquid
chromatography and capillary electrophoresis with different detection systems were used for determination
of aristolochic acids in various samples.
Results: In this review, different analytical methods have been discussed in brief and applications of
them in diverse samples have been summarized.
Conclusion: Different approaches are compared from point of sensitivity, selectivity, and extraction
efficiency.