Title:Progress in One-pot Bioconversion of Cephalosporin C to 7-Aminocephalosporanic Acid
Volume: 19
Issue: 1
Author(s): Qiang Tan*, Jie Qiu, Xiang Luo, Yewang Zhang, Yuting Liu, Yuquan Chen, Jing Yuan and Wei Liao
Affiliation:
- Institute of Pharmacy, Guangxi University of Chinese Medicine, Guangxi, 530001,China
Keywords:
One-pot, bioconversion, enzymatic process, cephalosporin C, 7-aminocephalosporanic acid, acylase.
Abstract: Background: Cephalosporins are the most widely used semisynthetic antibiotics, which
acted on bacterial cell wall (peptidoglycan) synthesis. The key intermediate for fabricating about twothirds
of cephalosporins in clinical use is 7-aminocephalosporanic acid (7-ACA), which is derived
from chemical or enzymatic deacylation of the natural antibiotic cephalosporin C (CPC). The chemical
deacylation process has been replaced by the enzymatic deacylation process because the chemical
process required harsh conditions and released toxic waste.
Methods: A two-step enzymatic process that utilized D-amino acid oxidase (DAAO) and 7-β-(4-
carboxybutanamido)-cephalosporanic acid acylase (GLA) for two successive reactions has been applied
for the conversion of CPC to 7-ACA in an industrial scale.
Results: To simplify the process and lower costs, the one-pot enzymatic processes were developed by
the application of the mono-enzymatic process (application of cephalosporin C acylase or the variants
of GLA), di-enzymatic process (simultaneous action of DAAO and GLA) or the tri-enzymatic process
(simultaneous action of DAAO, GLA and catalase) for direct conversion of CPC to 7-ACA.
Conclusion: Here, we mainly focused on the description of these one-pot enzymatic processes and
emphasized on the preparation of the involved biocatalysts.
