Title:α-Mangostin, A Natural Xanthone, Induces Apoptosis and ROS Accumulation in Human Rheumatoid Fibroblast-Like Synoviocyte MH7A Cells
Volume: 17
Issue: 5
关键词:
类风湿性关节炎，天然产物，MH7A细胞，凋亡，α-倒捻子素，活性氧积累。
摘要: Background: Rheumatoid arthritis (RA) is a complicated and heterogeneous
chronic disease with the characteristic of progressive joint destruction, deformity and
disability. It is associated with not only genetic but also environmental factors. Many
studies suggest that RA-derived fibroblast-like synoviocyte (RA-FLS) is involved in the
pathogenic process of RA. The apoptosis and proliferation of RA-FLS is of great
importance in the development and progression in RA. Nowadays, more and more
traditional Chinese medicine (TCM) or natural products are studied in the treatment of
RA.
Objective: To investigate the effects of several natural products and the apoptosisinduced
effect of α-mangostin and its underlying mechanisms in RA-FLS MH7A cells.
Methods: The effects of natural products on MH7A cells were detected by MTT assay.
Annexin V-FITC/PI double labeling and DAPI staining were adopted to observe the
apoptosis induced by α-mangostin. The apoptosis related proteins were measured with
western blotting analysis. ROS accumulation was determined by DHE staining.
Results: Xantones, including Garcinone C, α-mangostin and γ-mangostin, significantly
inhibited the MH7A cell viability. And α-mangostin induces apoptosis in MH7A cells.
Further study showed that α-mangostin increased the ratio of Bax/Bcl-2 and increased
the ROS generation in MH7A cells.
Conclusion: α-Mangostin induces the apoptosis of MH7A cells through increasing ROS
accumulation and the ratio of Bax/Bcl-2, suggesting that α-mangostin should be benefit
to the therapy of RA.
