Title:Maghemite Nanoparticle Induced DNA Damage and Oxidative Stress Mediated Apoptosis of CRL-1739 Adenogastric Carcinoma Cell
Volume: 8
Issue: 1
Author(s): Karthikeyan Manikandan, Amutha Santhanam*Setty Balakrishnan Anand
Affiliation:
- National Centre for Nanoscience and Nanotechnology, University of Madras, Guindy Campus, Chennai,India
Keywords:
Adenocarcinoma, anti-proliferative, apoptosis, G2/M phase, maghemite nanoparticle, normal
cells IEC-6.
Abstract: Background: Maghemite nanoparticle is well known for its biocompatibility
compared to other types of nanoparticles having huge biomedical applications. The aim
of this study was to investigate the significance of maghemite nanoparticle and analyse
the anti proliferative, oxidative stress and apoptosis potency of this on CRL-1739 Adenogastric
carcinoma (AGS) cell line in order to understand its antitumor effect on gastric
carcinoma.
Methods: The cytotoxicity was examined by MTT assay and microscopic analysis on
AGS cell line along with a small intestinal epithelial cell line IEC-6. The oxidative stress
and cell damage on AGS were monitored by measuring the anti oxidants of glutathione S
Transferase (GST), reduced glutathione (GSH) and trypan blue exclusion and Lactate dehydrogenase
release (LDH) assay respectively. In addition live/dead assay, DNA fragmentation
assay and semi quantitative PCR analysis were carried out.
Results: The cytotoxicity results showed that significant growth inhibition was observed in
the cancer cells compared to normal cells. The cytotoxicity resulted here was due to oxidative
stress that reflected in the reduction of antioxidants and elevated levels of ROS and
LDH. Moreover, it was investigated that nanoparticles arrested cell growth in the G2/M
phase transition. The cell death mechanism induced by the nanoparticle upregulated the
expression of apoptotic marker p53 along with down regulation of oncogene C-myc.
Conclusion: The result obtained in this study suggests that the maghemite nanoparticle
explode the activation of apoptosis in cancer cells by G2/M phase arrest as well as shows
to be a potent targeted therapeutic agent.
