Title:Liquid Chromatographic Determination of Malondialdehyde in Plasma Samples After Liquid–Liquid Microextraction
Volume: 14
Issue: 4
Author(s): Sajad Azizi, Jafar Soleymani, Maryam Khoubnasabjafari, Azam Samadi and Abolghasem Jouyban*
Affiliation:
- Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz,Iran
Keywords:
Malondialdehyde, 2, 4-dinitrophenylhydrazine, vortex assisted liquid liquid microextraction, N2 assisted liquid liquid
microextraction, validation, samples.
Abstract: Background: Malondialdehyde is a product of lipid peroxidation of polyunsaturated fatty
acids in foods and biological samples and widely used as biomarker of oxidative stress in various diseases.
We describe the validation of a new microextraction-LC-UV method for the determination of
malondialdehyde (MDA) in the form of 2,4-dinitrophenylhydrazine (DNPH) derivatization in plasma
samples.
Objective: A new microextraction technique called vortex and N2 assisted liquid-liquid microextraction
has been developed for the determination MDA in plasma samples.
Method: The DNPH-derivatized MDA was extracted into ethyl acetate as an extraction solvent and
measured by LC-UV at 310 nm after evaporation of ethyl acetate and re-dissolving in mobile phase
composed of 0.2% acetic acid–acetonitrile (50:50; v/v). This method validated according to U.S. Food
and Drug Administration guidelines.
Results: The limit of detection of MDA was 0.038 µmol L-1 (2.74 µg L-1) and the intra and interday
relative standard deviations were in the range of 3.8-5.0% and 5.5-9.4%, respectively for different concentrations
of MDA.
Conclusion: A precise and valid method to measure MDA as DNPH derivatization in plasma samples
using LC was proposed.