Title:Chondroitin Sulfate (CS) Lyases: Structure, Function and Application in Therapeutics
Volume: 19
Issue: 1
Author(s): Aruna Rani, Seema Patel and Arun Goyal*
Affiliation:
- Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam,India
Keywords:
Proteoglycans, glycosaminoglycans, chondroitin sulfate, chondroitin lyase, β-elimination, (α/α)5 toroid topology,
cell invasion, differentiation.
Abstract: Glycosaminoglycans (GAGs) such as chondroitin sulfate (CS) are the chief natural polysaccharides
which reside in biological tissues mainly in extracellular matrix. These CS along with adhesion
molecules and growth factors are involved in central nervous system (CNS) development, cell
progression and pathogenesis. The chondroitin lyases are the enzyme that degrade and alter the CS
chains and hence modify various signalling pathways involving CS chains. These CS lyases are substrate
specific, can precisely manipulate the CS polysaccharides and have various biotechnological,
medical and therapeutic applications. These enzymes can be used to produce the unsaturated oligosaccharides,
which have immune-modulatory, anti-inflammatory and neuroprotective properties. This review
focuses on the major breakthrough of the chondroitin sulfate degrading enzymes, their structures
and functioning mechanism. This also provides comprehensive information regarding production, purification,
characterization of CS lyases and their major applications, both established as well as emerging
ones such as neural development.
