Title:Stimulation of Laccase Biocatalysis in Ionic Liquids: A Review on Recent Progress
Volume: 19
Issue: 1
Author(s): Hao Liu*, Xing Wu, Jianliang Sun and Shicheng Chen*
Affiliation:
- State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou,China
- Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI,United States
Keywords:
Ionic liquids (ILs), laccases, activity, stimulation, stabilization, application.
Abstract: It has been well known that laccases can directly or indirectly catalyze oxidation of a broad
species of phenols, amines and many other electron donor substrates. However, laccases as biocatalyst
in “green” ionic liquids (ILs) media instead of conventional solvents are less known and regarded as an
innovative research direction. The presence of ILs can either inhibit or stimulate laccase activity,
strongly depending on water-miscibility and kosmotropic natures of ILs. In addition, enzyme source,
mediator, pH as well as water content are very important factors which influence laccase activity and
stability. Therefore, elucidation of mechanisms underlying the interactions between laccases and ILs
will facilitate to screen ILs with excellent laccase compatibility. Strategies based on molecular evolution,
enzyme immobilization and/or ILs modification greatly increase the tolerance of laccases against
specific ILs. The use of ILs can spread the laccase applications in fields of biosynthesis, biodegradation,
biosensor and biofuel cells. This article summarizes the recent progress, trends and problems in this field
and focuses, in particular, on the stimulation of laccase activity in aqueous ILs media.
