Title:Study on Utilization of Detoxified Jatropha curcas Seed Cake Subjected to Solid State Fermentation as a Dietary Supplement in Wistar Rats
Volume: 8
Issue: 3
Author(s): Belame S. Sharath, Sevva P. Muthukumar and Devappa Somashekar*
Affiliation:
- Department of Microbiology and Fermentation Technology, CSIR-Central Food Technological Research Institute, Mysore, Karnataka 570 020,India
Keywords:
Detoxification, Jatropha seed cake, Saccharomyces cerevisiae, solid-state fermentation, Wistar rats.
Abstract: Background: The presence of anti-nutrients and toxins like phorbol esters in
Jatropha curcas seed cake (JSC) limits its application in feeds. This study was done to assess the
potential of detoxified JSC as rat feed.
Methods: The rats were fed a diet containing 0-5 and 10% of detoxified fermented JSC for four
weeks. For the group I, only casein diet was used in rat feed as a negative control. For the group II,
untreated JSC was used in rat feed as a positive control. For the group III, fermented JSC using
Saccharomyces cerevisiae MTCC-36 was used. For the group IV, the fermented JSC treated with
65% ethanol to remove the residual toxic phorbol esters was used as rat feed.
Results: The rats fed with untreated JSC showed increased levels of serum liver enzymes as an indication
of the onset of liver disease resulting in mortality. In this group, rats died in week 2, confirming
that the cake is not safe as feed until it is processed. The rats fed with detoxified JSC with 5
and 10% level survived with no adverse effects, and the performance was on par with the control
groups, although the body weight was slightly less compared to control.
Conclusion: Therefore, it was concluded that the detoxified JSC might be the potential and alternative
source of protein in the animal feedstuffs up to 10% level. There are recent patents also suggesting
the use of alternative feed supplements in the animal feed applications.
