Title:Comparative Analysis of Flower Proteomes of Two Apple Genotypes Selected by their Different Resistance to Alternaria alternate
Volume: 13
Issue: 4
Author(s): Cai-xia Zhang, Li-yi Zhang, Yi Tian and Pei-hua Cong
Affiliation:
Keywords:
Apple flower, comparative proteomics, MALDI-TOF-TOF/MS, resistant, susceptible, two-dimensional electrophoresis.
Abstract: Background: Apple flowers can be used as a model material to study the disease resistance
of apple varieties. Our previous studies indicated that the resistance mechanisms of resistant
and susceptible cultivars may involve different defense response-related proteins.
Objective: To investigate the intrinsic differences between two previously introduced apple seedlings
under normal conditions.
Method: Apple flower samples from two seedlings (one resistant to Alternaria alternate and the
other susceptible) were analyzed using two-dimensional electrophoresis and matrix-assisted laser
desorption/ionization time-of-flight tandem mass spectrometry.
Results: These techniques identified 29 differentially accumulated proteins between the two samples,
which could be categorized into five classes. Among them, the most abundant accumulated defense
response proteins in the resistant apple included polyphenol oxidase, glycine-rich proteins, superoxide
dismutase and glutathione S-transferases, which might play crucial roles in the greater resistance
capacity of the resistant cultivar.
Conclusion: Herein, new information regarding the differences in resistance between two apple cultivars
was revealed. Considering the complex biology involved in the disease resistance mechanisms
in apples, further investigations may offer more insights into the molecular mechanisms of resistant
apple flowers. The data may also promote further investigations of target proteins.
