Title:Metabolomic Insight into Lipid and Protein Profile in Diabetes Using Mass Spectrometry
Volume: 16
Issue: 14
Author(s): Malgorzata Bukowiecka-Matusiak, Malgorzata Chmielewska-Kassassir, Dorota Szczesna and Lucyna A. Wozniak
Affiliation:
Keywords:
Diabetes, lipidomics, metabolomics, MS study, proteomics.
Abstract: In recent years, metabolomics has become a necessary tool for understanding the impact of
external and pathological factors on the operation of biological systems. The first reports of
metabolomics date back to the 1970s, however, the area only began to develop dynamically at the
beginning of this century and has proved effective only during the present decade. The five primary
tools used in this form of analysis are NMR spectrometry, HPLC, TLC-UV, GC-MS and LC-MS/MS,
with MS as the most universal approach, particularly when used together with chromatographic
separation and NMR. Diabetes mellitus type 2 (T2DM) is a rapidly growing problem with global
consequences. The metabolomic approach has been extensively applied to examining T2DM, insulin resistance and
obesity, not only to assess the development of the disease, but also to discover its potential biomarkers. The presented
review summarizes current studies on lipidomic and proteomic profiles in the context of different types of diabetes
mellitus disease (T1DM, T2DM and GDM), as determined by chromatography-coupled mass spectrometry.