Title:Biomimetic Mineralization of Tumor Targeted Ferromagnetic Iron Oxide Nanoparticles Used for Media of Magnetic Hyperthermia
Volume: 14
Issue: 3
Author(s): Siyu Xie, Guangfu Yin, Ximing Pu, Yang Hu, Zhongbin Huang, Xiaoming Liao, Yadong Yao and Xianchun Chen
Affiliation:
Keywords:
Active targetability, biomimetic mineralization, cytocompatibility, ferromagnetic Fe3O4 nanoparticles, magnetic
hyperthermia, tumor targeting peptide.
Abstract: Background: The magnetic hyperthermia has been recognized as a useful therapeutic modality
for malignant tumors, and IONPs have received a great deal of attentions for potential biomedical
applications. The aims of this paper are to design a biomimetic mineralization procedure to synthesize
the ferromagnetic and tumor targeting Fe3O4 nanoparticles, to conjugate bioactive molecule on particles,
to analyze properties of product.
Methods: IONPs were synthesized with the WSG-PF127 as the mineralization templates, which were
mixed by conjugating the peptide WSG on the surface of PF127. And the influence of different conditions,
such as templates, temperature, stirring speed on the particles was investigated.
Results: Above the critical micelle concentration (CMC), the catenulate PF127 molecules were assembled
into the hollow sphere-like micelle, and the morphology and size of the IONPs mineralized inside the hollow
cores of PF127 micelles could be controlled due to the space restricted effect. The saturation magnetization
was increased due to the higher crystallinity degree of the WSG-PF127-IONPs, the cytocompatibility
was improved by the WSG-PF127 wrapped around the IONPs, and the targetability was endowed via
the mediation of the peptide-WSG conjugated on hydrophilic segments of PF127 molecular chains.
Conclusion: The iron oxide nanoparticles with homogenous morphology, uniform size, and excellent
ferromagnetism have been successfully mineralized under the regulation of the PF127 micelles coupled
with the peptide-WSG. The improved ferromagnetism, the negligible cytotoxicity to HUVECs, and the
targetability to tumor cells of the biomimetically mineralized IONPs coupled with WSG-PF127 have
greater potential to be applied as the active tumor targeted media for magnetic hyperthermia.
