Title:Novel Methods of Genetic Modification of Human Pluripotent Stem Cells
Volume: 5
Issue: 2
Author(s): Maria V. Camarasa
Affiliation:
Keywords:
Genomic editing, naive pluripotent stem, patient-specific, pluripotent, stem cell/genetic therapy.
Abstract: Genomic engineering has enormous potential along basic research, drug discovery and cell
therapeutics. Many existing methods for targeted gene knockout mutagenesis or integration rely on
homologous recombination. The low rate of spontaneous recombination in nearly all mammalian cell
types, as well as the scale of screening, effort and time required to isolate the targeted events during
genome modification, have hindered progress in this field. The present review has the objective to
present latest improvements of technology to develop genetic modification to a clinical grade level so it can be used in
human therapy.
