Title:Understanding Cancer Drug Resistance by Developing and Studying Resistant Cell Line Models
Volume: 16
Issue: 3
Author(s): Cristina P.R. Xavier, Milica Pesic and M. Helena Vasconcelos
Affiliation:
关键词:
获得耐药性，抗癌药物，肿瘤耐药，肿瘤耐药细胞系，同基因细胞系，耐药机制，多重耐药
摘要: Despite the enormous number of anticancer drugs presently available in the clinic, treatment failure due to drug
resistance is very frequent. The identification of mechanisms of resistance to different drugs is necessary, in order to identify
ways to prevent and circumvent such resistance. Indeed, the identification of novel therapeutic targets to overcome cancer
drug resistance remains one of the major challenges in drug discovery and development. The methods employed to
identify drug resistance mechanisms and novel therapeutic targets depend greatly on the establishment of cancer drug
resistant cell lines. The establishment of such drug resistant cell lines is laborious and time-consuming and various
different approaches have been described. This manuscript reviews the methodologies that have been used to create
cancer drug resistant cell lines and to identify their mechanisms of resistance. In addition, this review highlights the most
frequent drug resistance mechanisms found in cancer cells.
