Title:Clinical Grade Stem Cell Culture
Volume: 5
Issue: 2
Author(s): Nandaraj Taye and Maria V. Camarasa
Affiliation:
Keywords:
Clinical grade, cytokines, embryonic stem cells, growth factors, induced pluripotent stem cells, matrix,
mesenchymal stem cells, stem cells, xeno-free.
Abstract: Use of animal products to culture human stem cells poses risk of pathogens and other
contaminations which may render stem cells therapeutically unsuitable. Hence, it is necessary to meet
the standards of good manufacturing practices, clinical-grade like and xeno-free conditions. The
protocol for culturing clinical grade stem cells needs to be modified so that maximum utilization can
be done for stem cell therapies. Modifications can be in the media compositions, additional growth
factors, small molecule compounds, and gene manipulations of the required transcription factors or the platform in which
stem cells can grow. After the clinical grade standards are met it needs the approval of FDA for use as transplantation
therapies. This review provides various methods and inventions for culturing clinical grade stem cells which have been
patented.
