Title:Identification of Drug Targets in Helicobacter pylori by in silico Analysis : Possible Therapeutic Implications for Gastric cancer
Volume: 16
Issue: 1
Author(s): Deepthi Nammi, Ravi C. P. K. Srimath-Tirumala-Peddinti and Nageswara Rao R. Neelapu
Affiliation:
Keywords:
Drug targets, genome analysis, host-pathogen comparison, proteome comparison, strain-strain comparison, speciesspecies
comparison.
Abstract: Helicobacter pylori colonize stomach, inducing gastritis, ulcers and gastric cancer. Drugs
are used to relieve pain, but not H. pylori infections. Hence, there is a need for discovery of drug
targets and drugs for H. pylori. An objective of this current study is to identify drug targets for H.
pylori. RAST was used to compare genomes of 23 H. pylori strains with Homo sapiens sapiens, other
Helicobacter species (H. acinonychis, H. hepaticus, H. mustalae) and among them, to identify 13471 unique genes.
Bacterial genes which are non-homologous to humans and essential for pathogen are identified using BLASTp. Later, 29
potential drug targets were identified by subjecting these genes to property analysis. Eleven of the 29 drug targets are
already experimentally validated, lending credence to our approach. These methods have enabled rapid identification of
drug targets with possible therapeutic implications for gastric cancer.
