Title:Physicochemical Characterization and Analgesic Effect of Inclusion Complexes of Essential Oil from Hyptis pectinata L. Poit Leaves with β-Cyclodextrin
Volume: 16
Issue: 5
Author(s): Paula dos P. Menezes, Adriano A. de S. Araujo, Grace Anne A. Doria, Lucindo J. Quintans-Junior, Makson G.B. de Oliveira, Marcio R.V. dos Santos, Juliana F. de Oliveira, Jivaldo do R. Matos, Flavio M. de S. Carvalho, Pericles B. Alves, Iara L. de Matos, Darlisson A. dos Santos, Ricardo N. Marreto, Gabriel F. da Silva and Mairim R. Serafini
Affiliation:
Keywords:
β-cyclodextrin, (E)-caryophyllene, essential oil, Hyptis pectinata, inclusion complexes.
Abstract: The formation of inclusion complexes of Hyptis pectinata essential oil (EOHP), with potent activities such as
anti-nociceptive, anti-inflammatory, among others, with β -cyclodextrin (β-CD), was obtained by slurry (SC) and paste
procedures (PC). The gas chromatography coupled to the mass spectrometry (GC/MS) analysis demonstrated a total of
36.4% monoterpenes and 63.6% sesquiterpenes in the EOHP. The major components of EOHP were identified as (E)-
caryophyllene (54.07%). The analysis of samples (PM, PC and SC) by GC/MS involved the surface and the total extracted
oils. The GC/MS results suggested important differences between in SC and PC methods indicating the complexation of
mono and sesquiterpenoids in different ratios. Furthermore, the thermal analysis techniques suggests the complexation,
especially in SC, which show a thermogravimetry/derivative thermogravimetry (TG/DTG) peak at 140-270ºC, probably
related to oil loss. Scanning electron microscopy (SEM) images showed reduction size of the samples mainly in the SC product.
Additionally, EOHP/ β-CD improves pharmacological profile of EOHP alone in formalin-induced pain protocol in mice.