Title:Capillary Electrophoresis Interfaced with a Mass Spectrometer (CE-MS): Technical Considerations and Applicability for Biomarker Studies in Animals
Volume: 15
Issue: 1
Author(s): Amaya Albalat, Holger Husi, Justyna Siwy, Jarlath E. Nally, Mark McLauglin, Peter D. Eckersall and William Mullen
Affiliation:
Keywords:
Biomarkers, CE-MS, farm animals, peptide profiling.
Abstract: Proteomics is a growing field that has the potential to be applied to many biology-related disciplines. However,
the study of the proteome has proven to be very challenging due to its high level of complexity when compared to genome
and transcriptome data. In order to analyse this level of complexity, high resolution separation of peptides/proteins are
needed together with high resolution analysers. Currently, liquid chromatography and capillary electrophoresis (CE) are
the two most widely used separation techniques that can be coupled on-line with a mass spectrometer (MS). In CE, proteins/
peptides are separated according to their size, charge and shape leading to high resolving power. Although further
progress in the area of sensitivity, throughput and proteome coverage are expected, MS-based proteomics have developed
to a level at which they are habitually applied to study a wide range of biological questions. The aim of this review is to
present CE-MS as a proteomic analytical platform for biomarker research that could be used in farm animal and veterinary
studies. This is a MS-analytical platform that has been widely used for biomarker research in the biomedical field but its
application in animal proteomic studies is relatively novel. The review will focus on introducing the CE-MS platform and
the primary considerations for its application to biomarker research. Furthermore, current applications but more importantly
potential application in the field of farm animals and veterinary science will be presented and discussed.
