Title:Synthesis and in vitro Cytotoxicity Studies of Certain Novel Heterocycles Derived from Bis 1, 2, 4-Triazoles and their DNA Damage Studies
Volume: 9
Issue: 8
Author(s): Madhusudan N. Purohit, Chandrashekhar Venkaraddi Mangannavar and Mayur C. Yergeri
Affiliation:
Keywords:
1, 2, 4-triazoles, 1, 3, 4-thiadiazoles, cytotoxicity, MTT assay, comet assay.
Abstract: Synthesis and evaluation of cytotoxicity of a series of heterocyclic compounds derived from 1, 4-bis-(5-
[hydrazinocarbonylmethylthio]-4-phenyl-1,2,4-triazol-3-yl) butane (1a-b) are described. The triazolo-triazoles (9-15) and
thiadiazoles (16-18) were prepared from respective thiosemicarbazide intermediates (2-8). The Schiff bases (19-24) were
prepared from (1a, b) by reacting with different carboxaldehydes in acetic acid medium. All the synthesized compounds
were characterized by IR, NMR and Mass spectral studies. The compounds were evaluated for in vitro cytotoxicity potential
using the standard MTT assay against a panel of three human cancer cell lines: Lung carcinoma A-549, Colon carcinoma
HT-29 and Breast Cancer MDA MB-231. The DNA damage activity of the compound 24 was evaluated by alkaline
comet assay.