Title:BACE1 Levels Correlate with Phospho-Tau Levels in Human Cerebrospinal Fluid
Volume: 10
Issue: 7
Author(s): Soraia Barao, Lujia Zhou, Katarzyna Adamczuk, Tine Vanhoutvin, Fred van Leuven, David Demedts, Anne-Catherine Vijverman, Xavier Bossuyt, Rik Vandenberghe and Bart De Strooper
Affiliation:
Keywords:
Alzheimer’s disease, BACE1, biomarker, human CSF, neurodegeneration, sandwich ELISA.
Abstract: Previous studies have investigated the activity and protein levels of BACE1, the β-secretase, in the brain and
cerebrospinal fluid (CSF) of Alzheimer’s disease (AD) patients, however, results remain contradictory. We present here a
highly specific and sensitive BACE1 ELISA, which allows measuring accurately BACE1 levels in human samples. We
find that BACE1 levels in CSF of AD patients and other neurological disorder (OND) patients are slightly increased when
compared to those of a non-neurological disorder control group (NND). BACE1 levels in CSF were well correlated with
total-tau and hyperphosphorylated tau levels in the CSF, suggesting that the recorded alterations in BACE1 levels correlate
with cell death and neurodegeneration.
