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Abstract
The interaction between proteins and sugars, termed as glycation has been a subject of increasing study over the past decade. The aim of the present study was to investigate the non-enzymatic interaction of thiol- proteinase inhibitor (cystatin) with three reducing sugars glucose, fructose and ribose. The behaviour of glycated cystatin was monitored by change in its hyperchromicity at 280nm, tryptophan fluorescence and Maillard fluorescence. The antipapain activity of glycated cystatin was found to be significantly lower than its non-glycated form. It was found that the incubation of cystatin with all the three sugars led to a parallel decrease in tryptophan fluorescence, enhancement in Maillard fluorescence and hyperchromicity in the UV-region. Among the three sugars studied ribose was found to be the most active in inducing structural and conformational alterations in the protein.
Keywords: Fructose, fluorescence, glycation, glucose, hyperchromicity, liver cystatin, maillard fluorescence, ribose, UV
Current Proteomics
Title:Spectral Methods of Characterizing the Conformational Changes of Glycated Goat Liver Cystatin
Volume: 9 Issue: 4
Author(s): Aaliya Shah, Mohd. Shahnawaz Khan, Medha Priyadarshini, Mohammad Aatif, Fakhra Amin and Bilqees Bano
Affiliation:
Keywords: Fructose, fluorescence, glycation, glucose, hyperchromicity, liver cystatin, maillard fluorescence, ribose, UV
Abstract: The interaction between proteins and sugars, termed as glycation has been a subject of increasing study over the past decade. The aim of the present study was to investigate the non-enzymatic interaction of thiol- proteinase inhibitor (cystatin) with three reducing sugars glucose, fructose and ribose. The behaviour of glycated cystatin was monitored by change in its hyperchromicity at 280nm, tryptophan fluorescence and Maillard fluorescence. The antipapain activity of glycated cystatin was found to be significantly lower than its non-glycated form. It was found that the incubation of cystatin with all the three sugars led to a parallel decrease in tryptophan fluorescence, enhancement in Maillard fluorescence and hyperchromicity in the UV-region. Among the three sugars studied ribose was found to be the most active in inducing structural and conformational alterations in the protein.
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Cite this article as:
Shah Aaliya, Shahnawaz Khan Mohd., Priyadarshini Medha, Aatif Mohammad, Amin Fakhra and Bano Bilqees, Spectral Methods of Characterizing the Conformational Changes of Glycated Goat Liver Cystatin, Current Proteomics 2012; 9 (4) . https://dx.doi.org/10.2174/157016412805219215
DOI https://dx.doi.org/10.2174/157016412805219215 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
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