Title:New Informatics and Automated Infrastructure to Accelerate New Leads Discovery by High Throughput Screening (HTS)
Volume: 16
Issue: 3
Author(s): Preston S. Donover, Marlin Yohn, Matthew Sim, Andrew Wright, Sandesh Gowda, Chip Allee, Amanda R. Schabdach and Melvin Reichman
Affiliation:
Keywords:
Automated sample retrieval system (ASRS), bio-repository, drug discovery, HTS, orthogonal pooling, sample
management, sample store
Abstract: The Lankenau Institute for Medical Research Chemical Genomics Center, Inc. has developed a new (patents
issued and pending) Nanotube Automated Repository System (NARS) for dynamic storage of millions of ‘single-shot’
samples stored in a new monolithic microtiter-storage tube plate of our own design we call ‘nanotubes.’ We have
integrated the NARS with customized software to efficiently access up to 10,000,000 samples stored continuously frozen
(–20°C) in a dehumidified enclosure and sealed in a new microtiter NARS plate that is SBS compliant. Additional
software was developed to analyze HTS data from orthogonally pooled compound libraries. Following ‘de-convolution’
of pooled HTS data, the software designates confirmatory retest samples to be ‘cherry-picked’ using the NARS. The
application of a new, fully-integrated infrastructure for new leads discovery is described in detail. Other applications for
our technologies and new infrastructure are discussed.
