Title: Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes
Volume: 8
Issue: 4
Author(s): S.A. Deepak, K.R. Kottapalli, R. Rakwal, G. Oros, K.S. Rangappa, H. Iwahashi, Y. Masuo and G.K. Agrawal
Affiliation:
Keywords:
Real-time PCR, applications, disease, microorganisms, pathogen, detection, quantification, plant-microbe interaction
Abstract: Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21st century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.