Title:Spectrophotometric Simultaneous Determination of Citral Isomers in Cyclodextrin Complexes with Partial Least Squares Supported Approach
Volume: 8
Issue: 4
Author(s): Diogo Miron, Fernanda Battisti, Carla Schwengber Ten Caten, Paulo Mayorga and Elfrides Eva Scherman Schapoval
Affiliation:
Keywords:
Citral, Cyclodextrins, Encapsulating Agents, Factorial Design, Geranial, HPLC, Isomers, Microencapsulation,
Neral, PLS, UV, Volatile Oils
Abstract: This article describes the development and optimization of a UV spectrophotometric method to evaluate the
isomers of citral in cyclodextrins complexes by a partial least squares (PLS) regression model. The central composite design
(CCD) associated with the response surface methodology (RSM) was applied to select the wavelength range that
provides the best prediction results. β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) complexes with
citral were prepared in ethanolic solution and spray dried. These samples were analyzed by an optimized PLS-UV model
and the results compared with HPLC-UV determinations to evaluate the predictive power of the PLS-UV model. The results
from the PLS-UV model showed a relative standard deviation of prediction (RSEP) ranging from 0.87% for neral up
to 1.42% for geranial. The relative standard deviation (RSD) obtained in analyses of four replicates of citral-cyclodextrins
complexes ranged from 2.2% to 4.1%. Geranial showed higher affinity than neral for both types of cyclodextrins evaluated.
Finally, β-CD was shown to be a better complexing agent for citral than HP-β-CD.
