Title:Ganoderma lucidum Polysaccharides Exert Anti-Hyperglycemic Effect on Streptozotocin-Induced Diabetic Rats Through Affecting β-Cells
Volume: 15
Issue: 7
Author(s): Jusheng Zheng, Bin Yang, Yinghua Yu, Qi Chen, Tao Huang and Duo Li
Affiliation:
Keywords:
Anti-hyperglycemic effect, apoptosis, Bax, Bcl-2, Casp-3, catalase, diabetes, Ganoderma lucidum, glutathione
peroxidase, iNOS, mRNA expression, metformin, pancreatic β-cells, PDX-1, polysaccharides, streptozotocin, superoxide dismutase
Abstract: Previous studies have demonstrated that Ganoderma lucidum polysaccharides (Gl-PS) exhibited potential antihyperglycemic
effect in rats. The aim of the present study was to investigate the mechanism of the hypoglycemic effect of
a low- molecular-weight Gl-PS in streptozotocin (STZ)-induced diabetic Sprague-Dawley (SD) rats. Gl-PS was extracted
and purified from Ganodema lucidum fruiting body. 50 male SD rats were included in the study; 10 were taken as healthy
controls; 40 were induced to diabetes by a single injection of 65 mg/kg STZ, of which 30 were selected as successful
diabetic rat models. The 30 diabetic rats were divided into three groups: Gl-PS (200 mg/kg Gl-PS), metformin (100 mg/kg
metformin) and diabetic control (n = 10 per group). After eight weeks’ oral administration, plasma concentrations of
fasting glucose, triacylglyceride, total cholesterol and nitric oxide were significantly decreased in Gl-PS and metformin
groups. Pancreatic superoxide dismutase, catalase and glutathione peroxidase were significantly increased in Gl-PS and
metformin groups. Histopathological results showed that Gl-PS and metformin had protective effect on β-cells. The
mRNA expressions of Bcl-2 and PDX-1 in pancreas were up-regulated, but Bax, iNOS and Casp-3 down-regulated in Gl-
PS and metformin groups compared to diabetic control group. The present results suggested that Gl-PS had a
hypoglycemic effect in STZ-induced diabetic rats through preventing apoptosis of pancreatic β-cells and enhancing β-
cells regeneration.