Title:Advances in Zebrafish High Content and High Throughput Technologies
Volume: 15
Issue: 7
Author(s): Filip Miscevic, Ori Rotstein and Xiao-Yan Wen
Affiliation:
Keywords:
Chemical biology, drug development, high content, high throughput screen, pharmacogenomics, system
automation, zebrafish, fully automated drug screening, Gene knockdown, Danio rerio
Abstract: The zebrafish has emerged as an excellent transitional screening model system between cell-based assays,
which are rapid and inexpensive but have limited physiological relevance, and higher vertebrate models, which have
better physiological relevance, but are more time-consuming and expensive to deploy. As vertebrates, zebrafish maintain
significant evolutionary proximity to humans and have been validated as robust models for drug research, studies of
mechanism and behavioral genetics. Unlike higher vertebrate models, zebrafish are well-suited to high-throughput
applications owing to their high fecundity, rapid extrauterine development and transparency during organogenesis
enabling in vivo labeling and imaging. Recent advances have been made in automating high content and high-throughput
zebrafish screens, with the goal of developing fully automated drug screening platforms. The application and continued
development of these technologies holds potential clinical significance in drug discovery and elucidating disease
mechanisms.
