Title:Effect of Bovine Serum Albumin on the Stability of Bicalutamide-Encapsulated Lipid Nano-Emulsion in Bovine Serum
Volume: 8
Issue: 2
Author(s): Shigehiko Takegami, Shoko Uchida, Eriko Aoi, Takumi Yamamoto, Aki Yasuhara, Madoka Fujihara, Kaori Hayashi and Tatsuya Kitade
Affiliation:
Keywords:
Lipid nano-emulsion, bicalutamide, bovine serum albumin, interaction, stability, sucrose palmitate
Abstract: Aiming at the development of a stable drug carrier for bicalutamide (BT), we prepared various lipid nano-emulsions (LNEs)
from a lipid mixture of soybean oil, phosphatidylcholine, sodium palmitate and sucrose palmitate (SP). These LNEs had mean particle
size of approximately 50 nm and zeta potential (ζ) of -40 to -30 mV and were investigated their stability in saline solution and in bovine
serum by using the dynamic light scattering method. In saline solution, while the droplet size of LNEs without and with BT reached more
than 150 nm, that of the BT-encapsulated LNEs with SP could be maintained within 100 nm even after 24 h. In contrast, the droplet size
of LNEs hardly increased even over 72 h placed in bovine serum, suggesting that bovine serum albumin (BSA) suppressed their coalescence.
The intrinsic fluorescence of BSA was quenched and blue-shifted in the presence of LNE. In addition, the ζ values of all LNEs increased
to around -20 mV according to the increase of BSA concentration. These results demonstrated that BSA interacted with LNE particles.
Furthermore, the fluorescence resonance energy transfer using a fluorescence probe revealed that not only BT encapsulated in LNE
particles but also SP strongly interacted with BSA.
