![conference banner](/images/conference-banner.jpeg)
Abstract
This review focuses on rapid, efficient, and sensitive characterization of the proteome by capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE) in conjunction with laser-induced fluorescence (LIF). A number of advanced CE-LIF and MCE-LIF techniques, including on-column concentration techniques, on-line chemical reaction and enzyme-assay techniques, integrated microdevices, and high-efficiency multidimensional separation techniques that have been developed within the past few years, are capable for the analysis of trace proteins in biological samples and/or in single cells. These powerful techniques also show great potential for drug screening and diagnosis. The basic principle of each technique and its advantages and shortcomings for proteomics studies are discussed in detail. We also highlight the importance of techniques that possess improved analytical sensitivity, sample throughput, and quantitation capabilities for identifying the complexities and dynamics of the proteome expressed by cells, tissues, or an organism.
Keywords: capillary electrophoresis, laser-induced fluorescence, microchip capillary electrophoresis, multidimensional separation, on-line concentration, proteomics, single cell
Current Proteomics
Title: Advanced Capillary and Microchip Electrophoretic Techniques for Proteomics
Volume: 1 Issue: 4
Author(s): Huan-Tsung Changa, Yu-Fen Huanga, Shin-Huei Chiou, Tai-Chia Chiu and Ming-Mu Hsieh
Affiliation:
Keywords: capillary electrophoresis, laser-induced fluorescence, microchip capillary electrophoresis, multidimensional separation, on-line concentration, proteomics, single cell
Abstract: This review focuses on rapid, efficient, and sensitive characterization of the proteome by capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE) in conjunction with laser-induced fluorescence (LIF). A number of advanced CE-LIF and MCE-LIF techniques, including on-column concentration techniques, on-line chemical reaction and enzyme-assay techniques, integrated microdevices, and high-efficiency multidimensional separation techniques that have been developed within the past few years, are capable for the analysis of trace proteins in biological samples and/or in single cells. These powerful techniques also show great potential for drug screening and diagnosis. The basic principle of each technique and its advantages and shortcomings for proteomics studies are discussed in detail. We also highlight the importance of techniques that possess improved analytical sensitivity, sample throughput, and quantitation capabilities for identifying the complexities and dynamics of the proteome expressed by cells, tissues, or an organism.
Export Options
About this article
Cite this article as:
Changa Huan-Tsung, Huanga Yu-Fen, Chiou Shin-Huei, Chiu Tai-Chia and Hsieh Ming-Mu, Advanced Capillary and Microchip Electrophoretic Techniques for Proteomics, Current Proteomics 2004; 1 (4) . https://dx.doi.org/10.2174/1570164043152777
DOI https://dx.doi.org/10.2174/1570164043152777 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Machine Learning Based Computational Biology and Digital Medicine
Machine learning has become a major research topic with a wide range of applications, such as intelligent driving, public security, financial risk management, medical diagnosis, and computational biology. This Special Thematic Issue entitled: "Recent Trends in Machine Learning Based Computational Biology and Digital Medicine" aims to explore the scientific-technological frontiers ...read more
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
![Wayfinder Image](/images/wayfinder.jpg)
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Lipoprotein (a) Levels and Abdominal Aortic Aneurysm. A Systematic Review
and Meta-analysis
Current Pharmaceutical Design Management of Hypertension in Relation to Acute Coronary Syndromes and Revascularisation
Current Pharmaceutical Design Consequences of Elevated Homocysteine During Embryonic Development and Possible Modes of Action
Current Pharmaceutical Design Heart Failure in Sub-Saharan Africa
Current Cardiology Reviews Formation of Mycobacterial Pellicles In Vitro and their Possible Impact on Tuberculosis Infection
Current Respiratory Medicine Reviews Treatment of Cardiovascular Calcification in Uremia
Current Vascular Pharmacology Pumping O2 with no N2: An Overview of Hollow Fiber Membrane Oxygenators with Integrated Arterial Filters
Current Topics in Medicinal Chemistry The Role of microRNA-126 in Vascular Homeostasis
Current Vascular Pharmacology Antiplatelet Agents in Cardiology: A Report on Aspirin, Clopidogrel, Prasugrel, and Ticagrelor
Current Pharmaceutical Design Fiber-Optic Technologies in Laser-Based Therapeutics: Threads for a Cure
Current Pharmaceutical Biotechnology Multiple, Extensive Cardiac and Pulmonary Hydatid Cysts Managed by a Single-stage Surgical Removal: A Case Report
New Emirates Medical Journal Carfilzomib: A Tale of a Heartbreaking Moment: Case Report and Concise Review of the Literature
Cardiovascular & Hematological Disorders-Drug Targets Aortic Stenosis, Aortic Regurgitation and Arterial Hypertension
Current Vascular Pharmacology MICA Molecules in Disease and Transplantation, a Double-Edged Sword?
Current Immunology Reviews (Discontinued) Heart Failure Models: Traditional and Novel Therapy
Current Vascular Pharmacology Biomarkers in Atrial Fibrillation; From Pathophysiology to Diagnosis and Treatment
Current Medicinal Chemistry Cardiac and Muscular Involvement in Idiopathic Inflammatory Myopathies: Noninvasive Diagnostic Assessment and the Role of Cardiovascular and Skeletal Magnetic Resonance Imaging
Inflammation & Allergy - Drug Targets (Discontinued) Bilirubin Chemistry and Metabolism; Harmful and Protective Aspects
Current Pharmaceutical Design EDITORIAL [Hot Topic: Biomarkers in Cardiovascular Disease (Guest Editor: Dimitris Tousoulis)]
Current Medicinal Chemistry Cardiac Resynchronization Therapy in Children
Current Cardiology Reviews