Abstract
Protein arrays with ability to simultaneously detect multiple protein expression levels, protein-protein interactions, protein-DNA or RNA interactions, protein-small molecule interactions and protein modifications will be an excellent tool in biomedical discovery. A variety of technologies have been developed to meet this need. In general, these assay systems can be divided into two major categories: label-based approaches, and sandwich or enzyme linked immunosorbent assay (ELISA)-based approaches. The purpose of this review is to provide an overview of the principles and techniques of ELISA-based protein arrays, with particular emphasis on the use of ELISA-based protein arrays for detection of multiple protein expression levels.
Keywords: protein arrays, antibody arrays, proteomics, elisa, protein expression, immunoassay, cytokine
Current Proteomics
Title: ELISA-based Protein Arrays: Multiplexed Sandwich Immunoassays
Volume: 1 Issue: 3
Author(s): Ruo-Pan Huang, Ying Lin, Li-Pai Chen, Weimin Yang and Ruochun Huang
Affiliation:
Keywords: protein arrays, antibody arrays, proteomics, elisa, protein expression, immunoassay, cytokine
Abstract: Protein arrays with ability to simultaneously detect multiple protein expression levels, protein-protein interactions, protein-DNA or RNA interactions, protein-small molecule interactions and protein modifications will be an excellent tool in biomedical discovery. A variety of technologies have been developed to meet this need. In general, these assay systems can be divided into two major categories: label-based approaches, and sandwich or enzyme linked immunosorbent assay (ELISA)-based approaches. The purpose of this review is to provide an overview of the principles and techniques of ELISA-based protein arrays, with particular emphasis on the use of ELISA-based protein arrays for detection of multiple protein expression levels.
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Cite this article as:
Huang Ruo-Pan, Lin Ying, Chen Li-Pai, Yang Weimin and Huang Ruochun, ELISA-based Protein Arrays: Multiplexed Sandwich Immunoassays, Current Proteomics 2004; 1 (3) . https://dx.doi.org/10.2174/1570164043379226
DOI https://dx.doi.org/10.2174/1570164043379226 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
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