Title: Assessment of Cryopreserved Hepatocytes as an Alternative to Fresh Hepatocytes forComparative InterspeciesMetabolism Studieswith Suitable Acceptance Criteria
Volume: 1
Issue: 2
Author(s): Ziqiang (Zack) Cheng, Christine E. Herron, Gary Bowers and Mark de Serres
Affiliation:
Keywords:
Cryopreserved hepatocytes, fresh hepatocytes, comparative metabolism, metabolite profiling, structural identification, radiochemical flow detection
Abstract: Fresh hepatocytes have been the choice for interspecies comparative drug metabolism studies. Cryopreserved hepatocytes represent a readily available alternative when combined with acceptance limits based on the metabolic turnover of 7-ethoxycoumarin. Results for the ten NCEs examined show that the metabolites formed were strongly correlated in fresh and cryopreserved hepatocytes.