Abstract
The anticancer activity of Ocimum basilicum extract and its fractions was evaluated using human cancer cell lines; active compound(s) residing in it were identified and mechanism of their anti-proliferative action was explored. Methanolic extract was fractionated into petroleum ether soluble (PE-S) and insoluble (PE-I) fractions. These were evaluated on HT-144, MCF-7, NCI-H460 and SF-268 cell lines using Sulforhodamine B assay. Immunofluorescence microscopy was employed to study their effects on the cytoskeleton and nuclei of MCF-7 cells. Fractionation of PE-I (GI50: 5 μg/ml; LC50: 71μg/ml against MCF-7) led to the isolation of four compounds, mainly ursolic acid (LC50: 18.6 μg/ml). Ursolic acid (100 μM) induced a significant decrease in the percentage of cells in anaphase/telophase stages along with F-actin aggregation and mitotic spindle distortion. These results support anti-proliferative activity of O. basilicum extract against MCF-7 cells which may partly be due to effects of ursolic acid on F-actin and microtubules.
Keywords: Anticancer, Cytoskeleton, MCF-7 cell line, Ocimum basilicum, Ursolic acid, petroleum ether soluble, PE-S, PE-I, HT-144, MCF-7, NCI-H460, SF-268, Betula alba, paclitaxel, vinblastine, Camptothecin acuminate, Podophyllum pel-tatum, Anvirzel, Nerium oleander, Oleanolic acid, 3-Epi-ursolic acid, 3-O-Methyl ursolic acid, DMSO, DAPI, SRB, trichloroacetic acid, trypan blue, tryp-sin-EDTA, MeOH, EtOAc, HPLC, photodiode array (PDA) detector, RPMI-1640, sulforhodamine B, Immunofluorescence Microscopy, Mitotic Index Assay, ANOVA, FITC channel, XTT assay, Jasplakinolide
Letters in Drug Design & Discovery
Title: Anticancer Activity of Ocimum basilicum and the Effect of Ursolic Acid on the Cytoskeleton of MCF-7 Human Breast Cancer Cells
Volume: 7 Issue: 10
Author(s): Kehkashan Arshad Qamar, Ahsana Dar, Bina S. Siddiqui, Nurul Kabir, Huma Aslam, Shakil Ahmed, Shaista Erum, Shazia Habib and Sabira Begum
Affiliation:
Keywords: Anticancer, Cytoskeleton, MCF-7 cell line, Ocimum basilicum, Ursolic acid, petroleum ether soluble, PE-S, PE-I, HT-144, MCF-7, NCI-H460, SF-268, Betula alba, paclitaxel, vinblastine, Camptothecin acuminate, Podophyllum pel-tatum, Anvirzel, Nerium oleander, Oleanolic acid, 3-Epi-ursolic acid, 3-O-Methyl ursolic acid, DMSO, DAPI, SRB, trichloroacetic acid, trypan blue, tryp-sin-EDTA, MeOH, EtOAc, HPLC, photodiode array (PDA) detector, RPMI-1640, sulforhodamine B, Immunofluorescence Microscopy, Mitotic Index Assay, ANOVA, FITC channel, XTT assay, Jasplakinolide
Abstract: The anticancer activity of Ocimum basilicum extract and its fractions was evaluated using human cancer cell lines; active compound(s) residing in it were identified and mechanism of their anti-proliferative action was explored. Methanolic extract was fractionated into petroleum ether soluble (PE-S) and insoluble (PE-I) fractions. These were evaluated on HT-144, MCF-7, NCI-H460 and SF-268 cell lines using Sulforhodamine B assay. Immunofluorescence microscopy was employed to study their effects on the cytoskeleton and nuclei of MCF-7 cells. Fractionation of PE-I (GI50: 5 μg/ml; LC50: 71μg/ml against MCF-7) led to the isolation of four compounds, mainly ursolic acid (LC50: 18.6 μg/ml). Ursolic acid (100 μM) induced a significant decrease in the percentage of cells in anaphase/telophase stages along with F-actin aggregation and mitotic spindle distortion. These results support anti-proliferative activity of O. basilicum extract against MCF-7 cells which may partly be due to effects of ursolic acid on F-actin and microtubules.
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Arshad Qamar Kehkashan, Dar Ahsana, S. Siddiqui Bina, Kabir Nurul, Aslam Huma, Ahmed Shakil, Erum Shaista, Habib Shazia and Begum Sabira, Anticancer Activity of Ocimum basilicum and the Effect of Ursolic Acid on the Cytoskeleton of MCF-7 Human Breast Cancer Cells, Letters in Drug Design & Discovery 2010; 7 (10) . https://dx.doi.org/10.2174/1570180811007010726
DOI https://dx.doi.org/10.2174/1570180811007010726 |
Print ISSN 1570-1808 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-628X |
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