Abstract
Alkaloids are a group of natural products with unmatched chemical diversity and biological relevance forming potential quality pools in drug screening. The molecular aspects of their interaction with many cellular macromolecules like DNA, RNA and proteins are being currently investigated in order to evolve the structure activity relationship. Isoquinolines constitute an important group of alkaloids. They have extensive utility in cancer therapy and a large volume of data is now emerging in the literature on their mode, mechanism and specificity of binding to DNA. Thermodynamic characterization of the binding of these alkaloids to DNA may offer key insights into the molecular aspects that drive complex formation and these data can provide valuable information about the balance of driving forces. Various thermal techniques have been conveniently used for this purpose and modern calorimetric instrumentation provides direct and quick estimation of thermodynamic parameters. Thermal melting studies and calorimetric techniques like isothermal titration calorimetry and differential scanning calorimetry have further advanced the field by providing authentic, reliable and sensitive data on various aspects of temperature dependent structural analysis of the interaction. In this review we present the application of various thermal techniques, viz. isothermal titration calorimetry, differential scanning calorimetry and optical melting studies in the characterization of drug-DNA interactions with particular emphasis on isoquinoline alkaloid- DNA interaction.
Keywords: Thermal techniques, ITC, DSC, thermal melting, isoquinoline alkaloids, DNA binding, Calorimetry, deoxyribonucleic acids, isothermal titration calo-rimetry, differential scanning calorimetry, benzylisoquinolines, protopines, benzo, phenanthridines, protoberberines, Protoberberi-nes, berberine, palmatine, coralyne, benzo[c]phenanthridine group, entropy contribution, heat capacity, Clostridium perfringens, Micrococcus lysodeikticus, Sanguinarine-DNA Complexation, Differential scanning calorimetric analysis, Berberine-DNA Complexation, Palmatine-DNA Complexation, Coralyne-CT DNA Complexation
Mini-Reviews in Medicinal Chemistry
Title: Isoquinoline Alkaloids and their Binding with DNA: Calorimetry and Thermal Analysis Applications
Volume: 10 Issue: 13
Author(s): Kakali Bhadra and Gopinatha Suresh Kumar
Affiliation:
Keywords: Thermal techniques, ITC, DSC, thermal melting, isoquinoline alkaloids, DNA binding, Calorimetry, deoxyribonucleic acids, isothermal titration calo-rimetry, differential scanning calorimetry, benzylisoquinolines, protopines, benzo, phenanthridines, protoberberines, Protoberberi-nes, berberine, palmatine, coralyne, benzo[c]phenanthridine group, entropy contribution, heat capacity, Clostridium perfringens, Micrococcus lysodeikticus, Sanguinarine-DNA Complexation, Differential scanning calorimetric analysis, Berberine-DNA Complexation, Palmatine-DNA Complexation, Coralyne-CT DNA Complexation
Abstract: Alkaloids are a group of natural products with unmatched chemical diversity and biological relevance forming potential quality pools in drug screening. The molecular aspects of their interaction with many cellular macromolecules like DNA, RNA and proteins are being currently investigated in order to evolve the structure activity relationship. Isoquinolines constitute an important group of alkaloids. They have extensive utility in cancer therapy and a large volume of data is now emerging in the literature on their mode, mechanism and specificity of binding to DNA. Thermodynamic characterization of the binding of these alkaloids to DNA may offer key insights into the molecular aspects that drive complex formation and these data can provide valuable information about the balance of driving forces. Various thermal techniques have been conveniently used for this purpose and modern calorimetric instrumentation provides direct and quick estimation of thermodynamic parameters. Thermal melting studies and calorimetric techniques like isothermal titration calorimetry and differential scanning calorimetry have further advanced the field by providing authentic, reliable and sensitive data on various aspects of temperature dependent structural analysis of the interaction. In this review we present the application of various thermal techniques, viz. isothermal titration calorimetry, differential scanning calorimetry and optical melting studies in the characterization of drug-DNA interactions with particular emphasis on isoquinoline alkaloid- DNA interaction.
Export Options
About this article
Cite this article as:
Bhadra Kakali and Suresh Kumar Gopinatha, Isoquinoline Alkaloids and their Binding with DNA: Calorimetry and Thermal Analysis Applications, Mini-Reviews in Medicinal Chemistry 2010; 10 (13) . https://dx.doi.org/10.2174/13895575110091235
DOI https://dx.doi.org/10.2174/13895575110091235 |
Print ISSN 1389-5575 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5607 |
Call for Papers in Thematic Issues
Bioprospecting of Natural Products as Sources of New Multitarget Therapies
According to the Convention on Biological Diversity, bioprospecting is the exploration of biodiversity and indigenous knowledge to develop commercially valuable products for pharmaceutical and other applications. Bioprospecting involves searching for useful organic compounds in plants, fungi, marine organisms, and microorganisms. Natural products traditionally constituted the primary source of more than ...read more
Computational Frontiers in Medicinal Chemistry
The thematic issue "Computational Frontiers in Medicinal Chemistry" provides a robust platform for delving into state-of-the-art computational methodologies and technologies that significantly propel advancements in medicinal chemistry. This edition seeks to amalgamate top-tier reviews spotlighting the latest trends and breakthroughs in the fusion of computational approaches, including artificial intelligence (AI) ...read more
Drugs and mitochondria
Mitochondria play a central role in the life and death of cells. They are not merely the center for energy metabolism but are also the headquarters for different catabolic and anabolic processes, calcium fluxes, and various signaling pathways. Mitochondria maintain homeostasis in the cell by interacting with reactive oxygen-nitrogen species ...read more
Mitochondria as a Therapeutic Target in Metabolic Disorders
Mitochondria are the primary site of adenosine triphosphate (ATP) production in mammalian cells. Moreover, these organelles are an important source of reactive oxygen and nitrogen species in virtually any nucleated cell type. The modulation of a myriad of cellular signaling pathways depends on the mitochondrial physiology. Mitochondrial dysfunction is observed ...read more
![](/images/wayfinder.jpg)
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Structures Required of Polyphenols for Inhibiting Advanced Glycation end Products Formation
Current Drug Metabolism Sperm Chromatin Protamination: An Endocrine Perspective
Protein & Peptide Letters A Novel Platinum-based Compound with Preferential Cytotoxic Activity against a Panel of Cancer Cell Lines
Anti-Cancer Agents in Medicinal Chemistry Costimulation, Coinhibition and Cancer
Current Cancer Drug Targets Curcumin Modulates Glycolytic Metabolism and Inflammatory Cytokines via Nrf 2 in Dalton’s Lymphoma Ascites Cells In Vivo
Anti-Cancer Agents in Medicinal Chemistry Endogenous Retroviruses in Domestic Animals
Current Genomics A Systematic Review of the Potential Herbal Sources of Future Drugs Effective in Oxidant-Related Diseases
Inflammation & Allergy - Drug Targets (Discontinued) Frailty of Older Age: The Role of the Endocrine - Immune Interaction
Current Pharmaceutical Design Bacterial Sepsis and Chemokines
Current Drug Targets subject Index To Volume 2
Current Cancer Drug Targets Old Wine in a New Bottle: The Warburg Effect and Anticancer Mechanisms of Resveratrol
Current Pharmaceutical Design Human Galectin-3 Selective and High Affinity Inhibitors. Present State and Future Perspectives
Current Medicinal Chemistry Anticancer Activity and DNA Binding of 4-Alkylenediamines Modified Naphthalimide Derivatives
Letters in Drug Design & Discovery The Gut Mucosa as a Site for Induction of Regulatory T-Cells
Current Pharmaceutical Design Cytotoxic T Cell Reponses Against Immunoglobulin in Malignant and Normal B Cells: Implications for Tumor Immunity and Autoimmunity
Current Pharmaceutical Design Peptidic Compound as DNA Binding Agent: <i>In Silico</i> Fragment-based Design, Machine Learning, Molecular Modeling, Synthesis, and DNA Binding Evaluation
Protein & Peptide Letters Molecular and Genetic Mechanisms of Osteoporosis: Implication for Treatment
Current Molecular Medicine The Structure and Function of Histone Deacetylases: The Target for Anti-cancer Therapy
Current Medicinal Chemistry NLRP3 Inflammasome Activation Leading to IL-1 – IL-17 Dependent Lung Inflammation and Fibrosis
Current Respiratory Medicine Reviews The Roles of Flavonols/Flavonoids in Neurodegeneration and Neuroinflammation
Mini-Reviews in Medicinal Chemistry