Title: Novel Antimicrobial Peptides Isolated from Skin Secretions of the Mexican Frog Hyla eximia
Volume: 16
Issue: 11
Author(s): Oscar Villa-Hernandez, Lorena Hernandez-Orihuela, Maria del Carmen Rodriguez, Fernando Zamudio-Zuniga, Ruben Castro-Franco, Victoria Pando and Cesar V.F. Batista
Affiliation:
Keywords:
Hyla eximia, antimicrobial peptides, mass spectrometry
Abstract: In this work, we describe the original characterization of peptides and proteins present in the skin secretions of the Mexican amphibian Hyla eximia. To this purpose, a novel water/dark extraction method, as well as the classic electrical stimulation procedure, was applied in order to extract the skin secretion. Two novel antimicrobial peptides He-1 and He-2 were sequenced. In addition, a molecular mass fingerprint revealed more than one hundred different molecules. Eight peptides in homogeneous form were assayed against five species of bacteria. Thereafter, the peptide He-2 demonstrated high antiparasitic activity against ookinete forms of malaria parasites at low concentration.
