Abstract
In this paper, we report our study of thermodynamic parameters of the interactions of antifreeze proteins (AFP) type I and it short segments with DMPC unilamellar vesicles as model for cell membrane. The heat of interactions between AFPs and the model cell membrane were studied by Isothermal Titration Calorimetry (ITC) at temperatures above and below phase transition temperatures of the membrane. It is shown that heat of interactions is linearly dependent on the temperatures below the phase transition of the membrane and constant at temperatures above phase. The heat of interaction above phase transition is assigned to the interaction of the AFP with the membrane, while below phase transition the ordering effect of the AFP influence the heat of interaction.
Keywords: Antifreeze proteins, model membrane, isothermal titration calorimetry, thermal hysteresis
Protein & Peptide Letters
Title: Isothermal Calorimetry Study of the Interactions of Type I Antifreeze Proteins with a Lipid Model Membrane
Volume: 17 Issue: 6
Author(s): Hagit Kun and Yitzhak Mastai
Affiliation:
Keywords: Antifreeze proteins, model membrane, isothermal titration calorimetry, thermal hysteresis
Abstract: In this paper, we report our study of thermodynamic parameters of the interactions of antifreeze proteins (AFP) type I and it short segments with DMPC unilamellar vesicles as model for cell membrane. The heat of interactions between AFPs and the model cell membrane were studied by Isothermal Titration Calorimetry (ITC) at temperatures above and below phase transition temperatures of the membrane. It is shown that heat of interactions is linearly dependent on the temperatures below the phase transition of the membrane and constant at temperatures above phase. The heat of interaction above phase transition is assigned to the interaction of the AFP with the membrane, while below phase transition the ordering effect of the AFP influence the heat of interaction.
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Cite this article as:
Kun Hagit and Mastai Yitzhak, Isothermal Calorimetry Study of the Interactions of Type I Antifreeze Proteins with a Lipid Model Membrane, Protein & Peptide Letters 2010; 17 (6) . https://dx.doi.org/10.2174/092986610791190354
DOI https://dx.doi.org/10.2174/092986610791190354 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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Plants are still the major repository of biologically active substances. In the last two decades, however, natural peptides and proteins of plant origin have gained increasing attention due to their pharmacological activities over a variety of human illness, including those mediated by infections and parasitosis and those involving different cellular ...read more
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