Title: Cloning and Expression of Human Beta-defensin-2 Gene in Escherichia Coli
Volume: 9
Issue: 1
Author(s): Xiangming Fang, Li Peng, Zhinan Xu, Jinmin Wu and Peilin Cen
Affiliation:
Keywords:
Human beta-defensin-2 (hBD-2), proinflammatory agents, anti-Gram-negative, transcription-polymerase chain reaction (RT-PCR), QIAprep Spin Minprep Kit, Chelating column, Coomassie brilliant blue G-250
Abstract: Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.
