Abstract
hSARS-CoV is the causative agent for SARS infection. Its spike glycoprotein (S) is processed by host furin enzyme to produce S1 and S2 fragments, the latter being crucial for fusion with the host membrane. This takes place via formation of a coiled coil 6-helix bundle involving N and Cterminal heptad repeat domains (HR-N and HR-C) of S2. Several fluorescent and non-fluorescent peptides from these domains were synthesized to examine their interactions by circular dichroism, thermal denaturation, native-page, mass spectrometry and fluorescence spectroscopy studies. Data revealed that HR-C domains (1153-1189), (1153-1172) and (1164-1184) all exhibit potent binding interactions with HR-N892-931 domain. These peptides may find useful therapeutic applications in SARS intervention.
Keywords: Severe acute respiratory syndrome, Spike protein, Heptad repeat domains, Host-virus fusion, Helical structure, Proteolysis
Protein & Peptide Letters
Title: Structural and Biochemical Investigation of Heptad Repeat Derived Peptides of Human SARS Corona Virus (hSARS-CoV) Spike Protein+
Volume: 15 Issue: 9
Author(s): Sarmistha Basak, Xiaolei Hao, Andrew Chen, Michel Chretien and Ajoy Basak
Affiliation:
Keywords: Severe acute respiratory syndrome, Spike protein, Heptad repeat domains, Host-virus fusion, Helical structure, Proteolysis
Abstract: hSARS-CoV is the causative agent for SARS infection. Its spike glycoprotein (S) is processed by host furin enzyme to produce S1 and S2 fragments, the latter being crucial for fusion with the host membrane. This takes place via formation of a coiled coil 6-helix bundle involving N and Cterminal heptad repeat domains (HR-N and HR-C) of S2. Several fluorescent and non-fluorescent peptides from these domains were synthesized to examine their interactions by circular dichroism, thermal denaturation, native-page, mass spectrometry and fluorescence spectroscopy studies. Data revealed that HR-C domains (1153-1189), (1153-1172) and (1164-1184) all exhibit potent binding interactions with HR-N892-931 domain. These peptides may find useful therapeutic applications in SARS intervention.
Export Options
About this article
Cite this article as:
Basak Sarmistha, Hao Xiaolei, Chen Andrew, Chretien Michel and Basak Ajoy, Structural and Biochemical Investigation of Heptad Repeat Derived Peptides of Human SARS Corona Virus (hSARS-CoV) Spike Protein+, Protein & Peptide Letters 2008; 15 (9) . https://dx.doi.org/10.2174/092986608785849173
DOI https://dx.doi.org/10.2174/092986608785849173 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
![](/images/wayfinder.jpg)
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Meet Our Editorial Board Member
Current Alzheimer Research Linking New Paradigms in Protein Chemistry to Reversible Membrane-Protein Interactions
Current Protein & Peptide Science White Matter Injury Mechanisms
Current Molecular Medicine Scientific Basis for the Use of Indian Ayurvedic Medicinal Plants in the Treatment of Neurodegenerative Disorders: 1. Ashwagandha
Central Nervous System Agents in Medicinal Chemistry Adjudin - A Male Contraceptive with Other Biological Activities
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery (Discontinued) The Multifactorial Nature of Alzheimer's Disease for Developing Potential Therapeutics
Current Topics in Medicinal Chemistry Association of Smoking and Alcohol Drinking with Dementia Risk Among Elderly Men in China
Current Alzheimer Research Glycoxidation and Wound Healing in Diabetes: An Interesting Relationship
Current Diabetes Reviews Metformin: A Growing Journey from Glycemic Control to the Treatment of Alzheimer’s Disease and Depression
Current Medicinal Chemistry Vasoactive Intestinal Peptide in Neurodevelopmental Disorders:Therapeutic Potential
Current Pharmaceutical Design Neuronal Vulnerability to Degeneration in Parkinson’s Disease and Therapeutic Approaches
CNS & Neurological Disorders - Drug Targets An Early Detection and Classification of Alzheimer's Disease Framework Based on ResNet-50
Current Medical Imaging Genomics and Pharmacogenomics of Brain Disorders
Current Pharmaceutical Biotechnology Cognition-Enhancing Drugs in Mild Cognitive Impairment (MCI) and Alzheimers Disease (AD): An Update [1]
Medicinal Chemistry Reviews - Online (Discontinued) Meet the Editorial Board
Current HIV Research The Hydrogen Peroxide and its Importance in Alzheimers and Parkinsons Disease
Current Medicinal Chemistry - Central Nervous System Agents Estrogen and Cytokines Production - The Possible Cause of Gender Differences in Neurological Diseases
Current Pharmaceutical Design A Second Look into the Oxidant Mechanisms in Alzheimers Disease
Current Neurovascular Research Pharmacological Inhibition of Platelet Reactivity. Clinical and Pharmacodynamic Effects
Current Vascular Pharmacology Neuroprotective Effects of Intravenous Anesthetics: A New Critical Perspective
Current Pharmaceutical Design