Abstract
Lipid storage protein 2 (Lsd 2) is a conserved insect protein that belongs to the small PAT family of proteins. PAT proteins are found associated to the lipid droplets of adipocytes and play significant roles in the regulation of triacylglycerides metabolism. Here we describe the expression and purification of Lsd2, its reconstitution in lipoprotein particles, the location of putative lipid binding sites and its secondary structure. This study provides the starting point for future studies on the mechanism of function of Lsd2. The similarities and differences between Lsd1 and Lsd2, the only PAT proteins found in insects, are discussed.
Keywords: Lipid storage droplet protein, adipocytes, fat body, Drosophila, lipolysis
Protein & Peptide Letters
Title: Purification and Characterization of Recombinant Lipid Storage Protein-2 from Drosophila melanogaster
Volume: 15 Issue: 9
Author(s): Estela L. Arrese, Laticia Rivera, Masakazu Hamada and Jose L. Soulages
Affiliation:
Keywords: Lipid storage droplet protein, adipocytes, fat body, Drosophila, lipolysis
Abstract: Lipid storage protein 2 (Lsd 2) is a conserved insect protein that belongs to the small PAT family of proteins. PAT proteins are found associated to the lipid droplets of adipocytes and play significant roles in the regulation of triacylglycerides metabolism. Here we describe the expression and purification of Lsd2, its reconstitution in lipoprotein particles, the location of putative lipid binding sites and its secondary structure. This study provides the starting point for future studies on the mechanism of function of Lsd2. The similarities and differences between Lsd1 and Lsd2, the only PAT proteins found in insects, are discussed.
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Cite this article as:
Arrese L. Estela, Rivera Laticia, Hamada Masakazu and Soulages L. Jose, Purification and Characterization of Recombinant Lipid Storage Protein-2 from Drosophila melanogaster, Protein & Peptide Letters 2008; 15 (9) . https://dx.doi.org/10.2174/092986608785849191
DOI https://dx.doi.org/10.2174/092986608785849191 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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