Title: Fused Rola Protein Enhances β-Glucoronidase Activity 50-Fold: Implication for Rola Mechanism of Action
Volume: 10
Issue: 3
Author(s): Leila Maria G. Barros, Rosane H. Curtis, Antonio Americo B. Viana, Laura Campos and Mauro Carneiro
Affiliation:
Keywords:
glucuronidase, rola protein, translational fusion, enzyme activity, protein thermal stability, protein accumulation
Abstract: We report that the plant oncoprotein RolA from Agrobacterium rhizogenes acts to stabilize β- glucoronidase (Gus) when the two proteins are expressed as a fusion protein in transformed tobacco. The observed 50-fold increase of Gus activity was shown to be related to protein accumulation, with no significant changes in mRNA abundance, kinetic properties of the enzyme and thermostability. The entire RolA sequence is essential to achieve the full effect since both the N-terminal region, spanning a putative reverse signal-anchor and nuclear targeting sequences, or the contiguous C-terminal portion were shown to increase Gus activity only 10-fold. A possible interference of RolA in the protein degradation pathway regulated by auxin is discussed.
