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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Ultrasensitive Staining-Free Protein Detection After PAA Gel Electrophoresis Using Deep UV Fluorescence

Author(s): Eugene Riaplov, Qiang Li and Stefan Seeger

Volume 14, Issue 7, 2007

Page: [712 - 715] Pages: 4

DOI: 10.2174/092986607781483787

Price: $65

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Abstract

We present the observation of separated protein bands after polyacrylamide (PAA) gel electrophoresis based on the staining-free detection of their ultra violet (UV)-induced fluorescence employing deep UV confocal fluorescence microscopy. Mixtures of the three biological compounds β-Galactosidase (from Escherichia coli), apo-Transferrin (bovine) and bovine serum albumin (BSA) have been separated and a staining free detection limit below 80 pg (7.0x108 molecules) per band has been achieved. This corresponds to ∼270 molecules in the detection volume for confocal microscopy.

Keywords: Native fluorescence, deep-UV, polyacrylamide, gel electrophoresis, staining free


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